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Home Results and reports Tech and sci description Impact and dissemination Consortium description Project management Contact

4.1 available research infrastructure, and its upgrade/development

University of Bucharest

UB-CESEC, UB-Lythos, UB-Bioch and (P2) IBB developed a complementary research infrastructure in an infrastructure project (www.infrabim.cesec.ro) with a Lab. of biogeochemistry (Faculty of Biology), a Lab. of biochemistry (Faculty of Chemistry) at Univ. of Bucharest, and a lab of sample preparation for metals analyses at P2 (IBB). At UB there is also a Lab. of geochemistry, and of paleomagnetism (Faculty of geology)

Laboratory of biogeochemistry and bioremediation

Single collector, quadrupole ICP-MS Perkin-Elmer ELAN DRC-e with axial field technology for trace elements, rare earth elements and isotopic analyses; multiwave 3000 microwave oven Anton Paar equipped with two rotors: 16HF100 with 16 high-pressure vessels made of ceramics (100 mL) and 8SXQ80 with 8 high-pressure vessels made of quartz (80 mL); XRF Thermo Scientific Niton XL3t environmental analyzer with GOLDD technology which represent the state-of-the-art for environmental applications allowing to perform rapid, non destructive data in situ on the spot screening for qualitative elemental identification; Kjeldahl system for nitrogen determination (Labortechnick Büchi AG) in soil and plant samples for environmental applications, composed of mineralized unit coupled with automatic scrubber and unit of analysis (Kjeldahl distiller coupled with automatic titrator);UV-VIZ spectrophotometer super Aquarius Cecil equipped with Scan, Time drive, Wavelength programs including enzymatic kinetic methods (Kin Lab), double fascicle, racks, Peltier system - thermoelectric controller and CE 572 fluorescence accessory. Plant growth chamber model LGC-5101 with humidity, light intensity and temperature controller. Liophilizator Martin Christ ALPHA 1-2LD+, analytical mill IKA M 20 with cooling system, shakes: magnetic with thermoelectric controller (Heidolph MR 3001 K), rotating (3040 GFL - used for sequential extractions), thermoelectric shake (BIOSAN, TS100) for microtubes, 25-100 0C and 250-1400 rpm and, open-air platform (MaxQ3000 Thermo scientific - for soil extraction nutrients) up to 20,000 rpm. Centrifuges Hettich (22R) with cooling system equipped with one angle rotor having 24 positions permitting the use of 1,5/ 2,2 ml Eppendorf tube and maximal up to 18000 rpm and the second interschimbable angle rotor having 12 positions for 15 ml standard tubes and maximal up to 6.000 rpm and without cooling system (Universal 320 R) equipped with one angle rotor having 8 positions for 50 ml standard tubes and maximal up to 6.000 rpm and the second angle rotor with 4 position for 100 ml quartz tube up to 6000 rpm.. Auto lab 4x4 Land Rover Defender for sampling and monitoring in terrestrial ecosystems. Water bath MEMMERT- WB/OB 7-4 (temperature range: 10-95 0C], ultrasonic bath RAYPA, UCI 75, up to 35 kHz and thermoelectric range 30-800C. SAM autoclave AUTROCOH A22 model with three sterilizer cycles in a temperature range: 121-1340C. Silent Crusher S ultraturax IKA T18 up to 75.000 rpm. Vortex Heidolph REAX TOP, up to 2400 rpm, analytical balance METTLER TOLEDO XS 204/M, semi-analytical balance Kern. Laboratory inoLab pH/ION 735 WTW, field multiparameter Multi 340i, WTW. Water distilling apparatus GFL 2001/4 and water purification system Sartorius arium 611VF. Freezer TENAK EL31LT.

Laboratory of mineralogy and geochemistry

X-ray diffractometer - x'pert Powder PANalytical θ-θ (ICDD database), X-ray fluorescence - XGT-7200 micro-XRF analyzer - HORIBA (Na - U), Grain size - Laser Diffraction Particle Size Distribution Analyzer HORIBA LA-950 (<2mm), IR Spectrometry - Jasco FT-IR-4000 (measurement wavenumber range 7800 to 350 cm-1), Olympus Microscope + video capture for research; Microscope - Nikon E600 POL, UV fluorescence microscopy - Hund 50FL, Stereographic Microscope - Nikon SMZ800, Microscope microspectrometer - Ocean Optics HR2000, Digital camera for microscope - Nikon Coolpix 950, Camera for fluorescence – Penguin Computer for digital image capture; Laboratory sample preparation and analysis: Oven roasting (max 30 samples, 1000° C); Oven for burning organic matter (max 6 samples, 600°C); Water distiller - Caloris D4; Oven with electronic control - Caloris E25; Electronic scale - Ohaus; Sieve-vibrator set for coarse grain size fraction; Set of 5 decanting tubes dams and 5 hydro-gauges + thermostate bath fine grain size fraction; Volumetric calcimeter – Eijkelkamp; Digital Ph meter with calibration solutions; Fixed angle centrifuge;

Laboratory for hydrogeological and biogeochemical modeling

UB-CESEC and UB-Hydro built a common modelling laboratory located at UB-Hydro with one server and one graphic station equipped with ARC-GIS, Statistica, Idrisi and Globalmapper, other desktop computers equipped with ARC-GIS.

Laboratory of Biochemistry and Bioremediation (LBB) belongs to the University of Bucharest, Faculty of Chemistry. Regarding the expertise in the project area, the research activities are well documented and supported by the last projects: “Molecular mechanisms involved in Saccharomyces cerevisiae cellular response to heavy metal and oxidative stress“ (PNII-IDEI), “Cellular and Molecular Biotechnologies for Medical Aplications“ (POS-DRU). The laboratory experience of the research team  includes: standard molecular biology methods (nucleic acid isolation/purification/quantitation, Southern, Northern, Western blotting, primers design, PCR gene analysis, gene fusion techniques), standard yeast and bacteria genetic techniques, cell imaging, microbial and mammalian cell cultures, transfection, RT-PCR, drug selection, drug tests (on yeast and mammalian cell cultures), kinetics of drug transport within cell, cation transport kinetics, yeast two-hybrid system, gene reporter assay, synthesis, extraction, purification and identification of natural compounds. The LBB infrastucture includes equipments for biochemistry, molecular biology and microbiology: clean room, growth chamber, hood with laminary flow, PCR termocyclers; real time_PCR; fluorescence/optical microscope; UV-Vis spectrometers; vertical and horizontal electrophoresis systems; gel documentation system; incubators (static and rotatory); heating mantles; centrifuges; freezer-80°C; autoclave, Flash Master Chromatograph, etc.

P1 (Prospecțiuni S.A.) hold 4x4 field cars, specialized boxes  and recipients for samples collecting and preserving, cooling equipment covering full range of analyses based on multiple methods, according with laboratory endowment: ICP-MS, ICP-OES, atomic absorption spectrometer, XRF difractometer, gas chromatograph, analytical and electronic scales, drying ovens and furnaces, agate grinder with balls.

P2 (IBB), beside the INRABIM infrastructure, implemented a major infrastructure project with structural funds which include full renovation of the buildings and the construction of a green houses and the purchasing of 8 growth chambers for vegetation.

One of the objectives of PNII Partnerships projects is to develop the infrastructure institutional capacity and human resource dedicated to applied research. The infrastructure will be developed during the TIMMAR lifetime as follows:

CO will improve the infrastructure for elements analyses with an optical emission spectrometer with nitrogen plasma. This is useful especially for fast and cheap determining the large concentrations of toxic and major elements in the highly contaminated soil and plant samples like those occurring in contaminated floodplains. Plasma optical emission spectrophotometer with nitrogen  - is a tool that provides a new technique for elemental analysis by atomic emission and is characterized by a microwave field coupled plasma (plasma nitrogen - consumes only air using a N2 generator) rapid sequence analysis, increased productivity through rapid sequential multielement measurements, very good detection limits and linear dynamic range expansion, detecting major, minor or trace elements and an increased tolerance to the matrix dissolved solids. Ensures lower operating costs by using air, eliminating the need of using gas as argon, acetylene, etc. and eliminates the need for spectral hollow cathode lamps. The instrument is able to improves safety by eliminating the need to use explosive gas cylinders and handling as well as safe using and unattended in "overnight".It will complement the existing ICP-MS, appropriate especially for toxic elements in traces and will serve all research centres involved in the project, with a special interest for geological samples analysed by Lythos research centre. Estimated price: 60000 Euros

P1 will purchase drilling equipment needed for sampling of tailing material. It will complement the existing drillers which can be used down to 6 m. Price: 20000 Euros

P2 will purchase equipments needed for the rapid plant determination of lab parameters needed for characterising the biomass abundances in plant communities. Thermal Hygrometer / programmable digital thermal balance determines under high performance soil/sediment moisture, is a portable automatic device with a digital camera and very easy to be use in field and laboratory, equipped with an infra-red dark radiation IDR (DARK infrared radiator) and an electronic system with very fast determination. Oven with natural convection equipped with three temperature scales up to 300° C, three classes of performance and thermal safety levels, and acoustic alarm overheating. Prices of both: 4500 Euros.

The improvement of human resources is underlined by the inclusion of post-docs, PhD students and MSc students (next chapter).